Notifications
Clear all

Disease Prevention Review of Medicinal Plants for Anti-Obesity Activity


Admin
Posts: 19
Admin
Topic starter
(@admin)
Member
Joined: 1 year ago

Review of Medicinal Plants for Anti-Obesity Activity

Satyajit Patra1*, S Nithya2, B Srinithya2 and Meenakshi SM2

1Division of Biochemistry and Genetics,American International MedicalUniversity, Saint Lucia, WI, USA

2Department of Biotechnology, Schoolof Chemical & Biotechnology, SASTRAUniversity,Thirumalaisamudram,Thanjavur, Tamilnadu, India – 613401

*Corresponding Author:
Dr. Satyajit Patra
Division of Biochemistry and Genetics
American International Medical University
Saint Lucia, WI, USA
Tel: 1-758-488-0322
Email: dr.patra@aimu-edu.us

Received Date: August 20, 2015; Accepted Date: December 01, 2015; Published Date: December 04, 2015

Citation: Patra S, Nithya S, Srinithya B, et al. Review of Medicinal Plants for Anti-Obesity Activity. Transl Biomed. 2015, 6:3. doi: 10.21767/2172-0479.100021

 
Visit for more related articles at Translational Biomedicine

Abstract

Obesity is a complex health issue to address, it is a serious and chronic disease that can have a negative effect on many systems in your body. Overweight and obesity may increase the risk of many health problems, including diabetes, heart disease, osteoarthritis and certain cancers. Obesity is increasing at an alarming rate throughout the world. Today it is estimated that there are more than 300 million obese people world-wide. Obesity is regarded as a disorder of lipid metabolism and the enzymes involved in this process could be targeted selectively for the development of antiobesity drugs. However, most of the anti-obesity drugs that were approved and marketed have now been withdrawn due to serious adverse effects. The naturopathic treatment for obesity has been explored extensively since ancient times and gaining momentum in the present scenario. Traditional medicinal plants and their active phytoconstituents have been used for the treatment of obesity and their associated secondary complications. Some active medicinal plants and their respective bioactive compounds were also tested by clinical trials and are effective in traemnet of obesity. This review focus on natural phytoextracts with their mechanism of action and their preclinical experimental model for further scientific research.

Keywords

Obesity; Antiobesity drugs; Medicinal plants

Introduction

In the present scenario, obesity is the major public health problem with about 1.9 billion adults (18 years and older) worldwide are overweight and about 600 million of them are clinically obese [1]. Obesity is characterized by increase in adipose cell size which is determined by amount of fat accumulated in the cytoplasm of adipocytes [2]. This change in the metabolism in the adipocytes is regulated by various enzymes such as fatty acid synthase, lipoprotein lipase and adipocyte fatty acid-binding protein [3].

Obesity results from an imbalance between energy intake and expenditure. It is caused by altered lipid metabolic processes including lipogenesis and lipolysis [4]. Lipogenesis is the process that stores free fatty acids in the form of triglyceride (TG) [5]; similarly, lipolysis is the process whereby the TG stored is metabolized to free fatty acids and glycerol [6]. Obesity accompanied by hyperlipidemia which is indicated by abnormally high concentration of lipids in blood [7]. The adipose tissue, an endocrine organ, has a major role in the regulation of metabolism and homeostasis, through the secretion of several biologically active adipokines [8]. During adipose tissue development, three major transcription factors, peroxisome proliferator-activated receptor (PPAR) γ, CCAAT/enhancer binding protein (C/EBP) α, and sterol regulatory element-binding protein (SREBP) 1c, regulate the expression of these lipid-metabolizing enzymes [9]. 5' AMPactivated protein kinase (AMPK) plays a major role in glucose and lipid metabolism by inactivating acetyl-CoA carboxylase (ACC) and stimulates fatty acid oxidation by up-regulating the expression of carnitine palmitoyltransferase-1 (CPT-1), PPARα, and uncoupling protein [10].

Nowadays, changes in human lifestyle and high energy diet have increased the incidence of obesity and even have become a risk factor to the population of children [11,12]. There are several pharmacologic substances available as antiobesity drugs, however they have hazardous side effects and hence natural products have been used for treating obesity in many Asian countries [13]. The potential of natural products for the treatment of obesity is still largely unexplored and can be an excellent alternative for the safe and effective development of antiobesity drugs [14].

Currently drugs available in the market for treatment of obesity can be divided into two major classes one being orlistat, which reduces fat absorption through inhibition of pancreatic lipase and the second is subutramine which is an anorectic or appetite suppressant. Both drugs have adverse effects including increased blood pressure, headache, drymouth, insomnia, and constipation [15,16]. In 1990 Fenfluramine and Dexfenfluramine were withdrawn from the market because of heart valve damage [17].

The US FDA in 1997 approved subutramine drug as a treatment for obesity. But later in October 2010 the drug was withdrawn from the market due to increased cardiovascular events and strokes [18,19]. In February 2011 the US FDA rejected approval of contrive which is a combination of bupropion/naltrexone due to concerns over potential cardiovascular risks [20]. Certain drugs have potential for abuse such as phentermine and diethylpropion and hence are approved for short term use [21].

At present, because of high cost and potentially hazardous side effects, the need for natural products against obesity is under exploration which may be an alternative strategy for developing effective, safe antiobesity drugs [22]. In 2000, Moro and Basile reported the use of certain well known medicinal plants that had claimed to be useful in treating obesity. The antiobesity effects of natural products from more diverse sources [23]. The aim of the present review was to update data on potential antiobesity herbal plants.

Methods

Databases used for this study to search include PubMed, Scopus, Google Scholar, Web of Science, and IranMedex with information reported between September 2, 2006 to September 22, 2014. Search of literatures was focused on human or animals investigating the benefits and harms of herbal medicines to treat obesity. The search terms were “obesity” and (“herbal medicine” or “plant”, “plant medicinal” or “medicine traditional”) without narrowing or limiting search items. Publications with abstract from the mentioned databases were used to prepare this review. The main outcome measures were defined as body weight, body fat, including fat mass/fat weight or fat percentage/visceral adipose tissue weight, waist or hip circumference, triceps thickness and appetite, and the amount of food/energy intake. Abstracts of publications on plants used to evaluate the activity on human, animals, cell lines studies with the main outcome as mentioned above were included. In vitro studies, review articles and letters to the editor were excluded. Two reviewers reviewed the articles for abstracts and title. Due to our inclusion and exclusion criteria, the duplicate articles were eliminated. The review includes active components and mechanism of action against obesity in animals and presented inTable 1. Some of the plants are tested for their activity against obesity in cell lines listed inTable 2and plants that were tested on human volunteers or clinical trails are listed inTable 3. In some instances scientist have evaluated the anti-obesity activity in isolated cell organelles, isolated cellular enzymes specifically pancreatic lipase are listed inTables 3 and 4respectively.Table 4present the plant which was studied for its activity against obesity in anin silicomodel.

 1Plant namePart(s)MechanismExperimental modelReference
1.Achyranthes asperaLinn
(Amaranthaceae)
SeedThe plant lowers total cholesterol, total triglyceride, and LDL-cholesterol, and increases HDL cholesterol level.High-fat-fed male Swiss albino mice[24]
2.Acorus calamusLinn
(Araceae)
Rhizome, roots and leavesEthyl acetate extarct ofA. calamusinhibits α-glucosidase activity.Glucose challenged mice.[25]
3.Achyranthes bidentataBlume (Amaranthaceae)RootThe drug affects on differentiation of adipocyte and decrease of phospho-Akt expression.Male Sprague-Dawley fed with a high-fat diet[26]
4.Actinidia polygamaMax
(Actinidiaceae)
FruitsSerum levels of aspartatedecreased in the mice treated with the extract without changes in serum levels of alanine transaminaseblood urea nitrogen and creatinine.Mice with high-fat dietinducedobesity[27]
5Adenophora triphyllaHara
(Campanulaceae)
RootAnti-obesity effect ofA.triphyllais mediated by increasing adipocytesadiponectin and activating pathway like AMPK, and PPAR-α, and decreasingadipokines TNF-α, GPDH, and PPAR-α. It also actively expresses low-density lipoprotein [LDL] receptor and cholestorl 7α- hydroxylase (CYA7A1) and inhibitsexpression of 3 hydroxy-3 methyl glutaryl - CoA (HMG-CoA)reductase.High fat diet (HFD)- C57B2/6 mice[28,29]
.6Aegle marmelosLinn (Rutaceae)LeavesThe active chemical constituents ofA.marmelosfor anti-adipogenic activity are halfordinol, ethyl ether aegeline and esculetin were responsible for the decrease in adipocyte accumulation. Active compounds umbelliferone and esculetin depletes lipid content in the adipocytes and by decreasing the hyperlipidemia.High fat diet induced obese male Sprague Drawly rat[30,31]
7Allium cepaLinn
(Amaryllidaceae)
PeelThe mRNA levels of activating protein (AP2) is down-regulated byA.cepaand those of carnitine palmitoyl transferase-1 α (CPT-1α) and fatty acid binding protein 4 (FABP4) are up-regulated. It is also proposed thatA. cepaincreases level of PPAR-γ2 mRNA (mesenteric fats) and IL-6 mRNA levels (perirenal and mesenteric fats).High fat-fed rats, Diet-induced obese Male Sprague-Dawley rats[32,33]
8Allium fistulosumLinn
(Liliaceae)
RootSignificant reduction in body weight and adipose tissue weight as well as adipocyte size. Genes involved in lipogenesis are down-regulated byA. fistulosum.High fat diet- induced mice[34]
9Allium nigrumLinn (Amaryllidaceae)BulbExtract ofA. nigrumupregulates AMPK, FOXO1, Sirt1, ATGL, HSL, perilipin, ACO, CPT-1, and UCP1 in the adipose tissues, whereas it downregulates CD36.High-fat diet induced obese mice[35]
10Allium sativumLinn
(Amaryllidaceae)
Stem, Bulb and RootsIt increases antioxidant enzymes and suppresses glutathione depletion and lipid peroxidation in hepatic tissue. Oil isolated fromA. sativumdown regulates sterol regulatory element binding protein-1c, acetyl-coA carboxylase, fatty acid synthase, and 3-hydroxy-3- methylglutaryl-coenzyme A reductase.High-fat diet-induced obese C57BL/6J mice[36,37]
11Alpinia galangaLinn
(Zingiberaceae)
RhizomeGalangin, the principal compontent ofA. galangaldecreases serum lipids, liver weight, lipid peroxidation and accumulation of hepatic TGs.Obesity induced in female rats by feeding cafeteria diet[38]
12Alpinia officinarumHance (Zingiberaceae)RootThe drug controls and improves lipid profile in animals by lowering serum Total-C, TG, and LDL-C concentrations, leptin content.Obesity in mice fed a high-fat diet[39,40]
13Angelica gigas
Nakai
(Apiaceae)
RootsDecursin, the active constituent ofA.gigasimproves glucose tolerance. Decursin along with the HFD significantly reduces secretionadipocytokines such as leptin, resistin, IL-6 and MCP-1.Mice fed a high-fat diet[41]
14Argyreia nervosaBojer
(Convolvulaceae)
RootSerum contents of leptin, total cholestrol, LDL, and triglycerides are reduced byA. speciosa.Diet- induced obesity rats[42]
15Artemisia iwayomogi
(Compositae)
Whole PlantIt downregulates adipogenic transcription factors PPARγ2 and C/EBPα and their target genes CD36, aP2, and FAS. The extract decreases gene expression of proinflammatory cytokines includingTNFα, MCP1, IL-6, IFNα, and INFβ in epididymal adipose tissue and reduces plasma levels of TNFα and MCP1.Mice fed a high-fat diet.[43]
16Atractylodes lancea(Thunb.) DC
(Compositae)
RhizomeItinhibits human pancreatic lipase. A new polyacetylene,syn-(5E,11E)-3-acetoxy-4-O-(3-methylbutanoyl)-1,5,11-tridecatriene-7,9-diyne-3,4-diol has been isolated and identified and exhibits lipase inhibitory activity.High-fat diet-induced obesity mice[44]
17Aster pseudoglehni Lim, Hyun & Shin
(Asteraceae)
LeavesIt suppresses expression of adipogenesis-related genes including PPARγ, C/EBPα, andSREBP1c.High fat diet induced-male C57BL/6J mice[45]
18Bauhinia variegataLinn
(Leguminosae)
Stem and root barksExtract ofE. variegataincreases brain serotonin level and high-density lipoprotein with a concomitant decrease in total cholesterol, triglycerides and low-density lipo protein.Hypercaloric diet -induced mice[46]
19Bergenia crassifolia(L.) Fritsch
(Saxifragaceae)
LeavesGalloylbergenin derivatives 3,11-Di-O-galloylbergenin and 4,11-
di-O-galloylbergenin are found to be present inB. crassifoliamoderates anti-lipid accumulationactivities.
Rats with high-calorie diet-induced obesity[47]
20Boehmeria nivea
(L.) Gaudich
(Urticaceae)
LeafThe extracts reduces adipose tissue weight serum alkaline aminotransferase and lactate dehydrogenase activities. Serum triglyceride,total cholesterol, LDL-cholesterol level, atherogenic index and cardiac risk factors are decreasedin animals fed with leaf powder and serum HDL -cholestrol levels are increased.High fat/ cholestrol diet- induced Male Sprague-Dawley rats.[48]
21Boerhaavia diffusaL.
(Nyctaginaceae)
RootThe phytoconstituents compounds sitosterol found in this plant which is structurally similar to cholesterol has beensuggested to reduce cholesterol by lowering the level of LDL-cholesterol and cholesterol level decreasedsignificantly in plasma without any side effects.High fat diet infemale Sprague-Dawley rats[49]
22Bombax ceibaL.
(Malvaceae)
Stem barkThe extract and active constituent gemfibrozil reverses the effects of HFD treatment on serum parameters. This activity may be due to the inactivation of acetyl-coA carboxylase, as a result of AMPK activation that mediates thermogenesis and FAS inhibition.Male, Wistar albino rats[50]
23Anredera cordifolia(Ten.) Steenis
(Basellaceae)
LeavesThe extract suppresses lipid accumulation and down-regulatesPPARγ, CCAAT/enhancer binding protein α, SREBP, and their target genes. It also increases phosphorylation of AMPK.High-fat diet-induced obese rats[51]
24Brassica rapaL.
(Brassicaceae)
RootLipolysis-related genes includingβ3-adrenergic receptor,hormone-sensitive lipase,adipose triglyceride lipase, anduncoupling protein are induced in white adipocytes of animals treated with extract ofB. campestris.High fat diet induced mice[52]
25Buddleja officinalisMaxim
(Scrophulariaceae)
Whole PlantThe extract reducesbody weight gain induced through adipocyte differentiation.High-fat diet to C57BL/6 mice[53]
26Bursera grandiflora(Schltdl.) Engl
(Burseraceae)
RootsB.grandifloraexerts anti-obesity activity by decreasing in the plasma-triglyceride levels.Mice of the C57B1/6 strain withhypercaloric diet.[54]
27Calanus finmarchicus
(Calanidae)
WaxC.finmarchicusreduces macrophage infiltration and downregulates expression of proinflammatory genes including tumor necrosis factor-α, interleukin–6, and monocyte chemoattractant protein–1, whereas up-regulates adiponectin expression.C57BL/6J mice with high-fat diet[55]
28Camellia japonicaL.
(Theaceae)
LeavesC.japonicacontrol insulin which is a modulator of lipid synthesis via sterol regulatory elementbinding protein-1c (SREBP-1c), decreased levels of insulin affects hepatic triglyceride synthesis.High fat diet induced Sprague−Dawley rats[56]
29Camellia oleifera Abel
(Theaceae)
Fruit hullSerumlevelsof total cholesterol and triacylglycerols are decreased but high-density lipoprotein cholesterol increased.Activity of fatty acid in animal liver is lowered by.Male ICR mice were fed a HFD[57]
30Camellia sinensis(L.) Kuntze
(Theaceae)
Leaves, twigs and stems, flower budsC. sinesisattenuates the gene expression of (SREBP-1c), fatty acid synthase and CCAAT/enhancer binding protein α. Extract found to reduce sICAM-1 release followed by nonpharmacological HGTE supplementation in db/db mice causing no adiponectin-inducing or antiadipogenic effects, reduced sICAM-1 release. Chakasaponin II from flower bud, suppresses mRNA levels of neuropeptide Y (NPY). The mRNA levels of adipogenic genes such as PPAR-γ, C/EBP-α, SREBP-1c, adipocyte fatty acid-binding protein, lipoprotein lipase and fatty acid synthase are decreased inC. Sinensistreated animals.Albino rats fed on high-fat diet, diet-induced obesity in Female ddY mice, high fat induced- C57BL/6J-Lepob/ob mice, high fat diet- induced C57BL/6J mice[58-64]
31Cheilanthes albomarginataC.B. Clarke
(Pteridaceae)
RhizomeExtract ofC.albomarginatalowers plasma triglyceride activity as well as reduces weight of adipose tissue.High fat diet induced obese male Sprague Dawleyrats[65]
32Chenopodium quinoaWilld
(Amaranthaceae)
SeedsC.quinoaextractattenuate mRNA levels of several inflammation markers including monocyte chemotactic protein-1, CD68 and insulin resistance osteopontin, plasminogen activator inhibitor-1 and it also reverses the effects of HF-induced downregulation of the uncoupling protein(s) mRNA levels in muscle.Mice fed with standard low-fat or a high-fat diet[66]
33Cirsium brevicauleA. Gray
(Compositae)
LeavesC.brevicauleinhibits fatty acid synthase and suppress the differentiation and lipid accumulation and affecting transcription factors such as SREBP-1c, C/EBPα, and PPARγ known to control the fatty acid synthase expression.C57BL/6 mice that were fed a high-fat diet[67]
34Citrus reticulataBlanco
(Rutaceae)
PeelmRNA expression levels of lipogenesis rrelated genes such as SREBP1c, FAS and ACC1 in the liver are lowered and the size of adipocytes are reduced.High fat diet induced mice[68]
35Citrus sunki(Hayata) Yu.Tanaka
(Rutaceae)
PeelPhosphorylation levels of AMPK andacetyl-CoAcarboxylase are decreased.High-fat dietinduced obese C57BL/6 mice[69]
36Clerodendrum phlomidisL. f.
(Lamiaceae)
RootsIt nhibits pancreatic lipase activity. The extract contains β-sitosterol.High fat diet induced obesity in C57BL/6J mice[70]
37Coccinia grandis(L.) Voigt
(Cucurbitaceae)
FruitReduces body weight, food intake, organ and fat pads weight and serum GLU, CHO, TRG, LDL and VLDL cholesterol levels and increases HDL levels.Cafeteria diet and Atherogenic dietinduced obesity in female rats.[71]
38Codonopsis lanceolata(Siebold & Zucc.) Benth. & Hook.f. ex Trautv
(Campanulaceae)
RootsReduces weight of adipose pads and the serum levels of triglycerides, total cholesterol, and low density lipoprotein cholesterol.High-calorie/high-fat-diet induced obesity Sprague-Dawley male rats[72]
39Coffea arabicaL.
(Rubiaceae)
SeedC.arabicadiet supplementation can impair glucose tolerance, hypertension, cardiovascular remodeling, and nonalcoholic fatty liver disease.High-carbohydrate, high-fat diet-fed Wistar male rats
40Coleus forskohlii(Willd.) Briq.
(Lamiaceae).
RootC.forskohliiact as anti-obesisity drug by inhibiting dyslipidemia.Diet-induced obesity in rats
41Corchorus olitoriusL.
(Malvaceae)
LeavesLiver tissue gene expression of gp91phox (NOX2) involved in oxidative stress is down-regulated byC.olitoriusand genes related to the activation of β-oxidation like PPARα and CPT1A are up-regulated by the plant.High fat diet - induced LDL receptor deficient mice
42Cordia ecalyculataVell
(Boraginaceae)
Whole plantAnti-obesity activity of theC.ecalyculatais medicated by anorectic central action, facilitating binding to adenosine receptors, thereby promoting an extension of adrenalin.Mice (albino, swiss strain) treated with cyclophosphamide
43Cornus officinalisSiebold & Zucc.
(Cornaceae)
RhizomePlatycodin D is the major component effective to activate AMPK-α. The extract reduces serum levels of aspartate transaminaseand alanine transaminase.C57BL/6J mice were fed a HF diet
44Cucumis meloL.
(Cucurbitaceae)
Fruit peelC.meloreduces gain in body weight, serum lipid profile like total cholesterol, triglyceride, LDL-C level, atherogenic index and increases serum HDL-C levels.High cholesterol diet induced in rats
45Cyamopsis tetragonoloba(L.) Taub
(Leguminosae)
BeansIt decreases adipose triglyceride accompanied by enhancing activity of hormone-sensitive lipase-facilitating mobilization of depot fat.High-fat-fed Wistar rats
46Dimocarpus longansLeenh
(Sapindaceae)
FlowerBy combined effect of decreased exogenous lipid absorption, normalization of hepatic PPAR-γ gene expression, suppression of pancreatic activity and SREBP-1c and FAS gene expression, and higher fecal triglyceride output.Hyper caloric diet- male Sprague-Dawley rats.
47Dioscoreae tokoronisLinn
(Dioscoreaceae)
RootIt decreases triglyceride, total plasma cholesterol, and low-density lipoprotein-cholesterol.It suppresses the expression of SREBP-1 as well as that of fatty acid synthase in adipose and liver tissues.High fat diet - induced mice
48Eucommia ulmoidesOliv
(Eucommiaceae)
Leaves, BarkAsperuloside increases adenosine5′-triphosphate production in WATand increases use of ketone bodies/glucose in skeletal muscle.Obesity induced by ovariectomy in female Wistar rats, rats fed a high-fat diet.
49Fraxinus excelsiorL.
(Oleaceae)
SeedSecoiridoids present enhances fat metabolism through β-oxidation, inhibit adipocyte differentiation during animal growth and limit fat accumulation.High fat diet induced mice
50Garcinia cowaRoxb. ex Choisy
(Clusiaceae)
Fruit, commercially available tabletInhibits the enzyme ATP-dependent citrate lyase, which catalyzes the cleavage of citrate to oxaloacetate and acetyl-CoA.Serum apo A1 levels are increased by the plant and the serum total cholesterol levels.Female Sprague-Dawley rats fed atherogenic diet
51Geranium thunbergii
Siebold ex Lindl. & Paxton
(Geraniaceae)
LeafThe extract ameliorates high-fat diet-induced obesity by altering the adipokine levels and downregulates expression of transcription factors and lipogenic enzymes involved in lipid metabolism.High fat diet - induced mice
52Glycine max(L.)Merr.
(Leguminosae)
BeanReductions glucose-6-phosphate dehydrogenase, malic enzyme, fatty acid synthetase, as well as acetyl-CoA carboxylase. The extract decreases appetite and HF diet-induced body weight gain through leptin-like STAT3 phosphorylation and AMPK activation.Diet-induced obese mice
53Gymnema sylvestre(Retz.) R.Br. ex Sm
(Apocynaceae)
LeavesInhibits serum lipids, leptin, insulin, glucose, apolipoprotein B and LDH levels while it increases the HDL-cholesterol, apolipoprotein A1 and antioxidant enzymes levels.High fatdiet-inducedobesity in wistar rats
54Hibiscus cannabinusL.
(Malvaceae)
LeavesIt decreases serum cholesterol, triglycerides, LDL-C, SGOT and SGPT activities.High cholesterol diet induced obesity in female albino rats
55Hibiscus sabdariffaL.
(Malvaceae)
LeafPromotes LXRα/ABCA1 pathway, stimulating cholesterol removal from macrophages, delaying atherosclerosis. Also, the extract treatment attenuated liver steatosis, downregulated SREBP-1c and PPAR-γ, blocked the increase of IL-1, TNF-α mRNA and lipoperoxidation and increased catalase mRNA.High fat diet-induced obese C57BL/6NHsd mice
56Holoptelea integrifolia(Roxb.) Planch.
(Ulmaceae)
BarkHMG-CoA reductase activity is reducedand cholesterol biosynthesis and increase in lecithin, cholesterolacyltransferaseactivity.Diet-induced obese rat
57Humulus lupulusL.
(Cannabaceae)
Female inflorescenceHepatic fatty acid synthesis is reduced through the reduction of hepaticSREBP1cmRNA expression in the rats fed a high-fat diet.High-fat diet induced obese rat, male C57BL/6J mice fed a HF diet
58Hunteria umbellata(K.Schum.) Hallier f.
(Apocynaceae)
SeedThe extract reduces weight gain pattern and causes dose related reductions in the serum lipids, Coronary artery risk index.Also,pre-treatment significantly improves triton-induced hepatic histological lesions.High fat diet- induced rats
59Hypericum philonotis
Schltdl. & Cham.
(Hypericaceae)
LeavesDecreases body weight and serum glucose levels. It also decreases total cholesterol, triglycerides and high-density lipoprotein-cholesterol without changing low-density lipoprotein-cholesterol, AI, AST and ALT level.Male Wistar rats fed with high fat diet
60Hypericum silenoidesJuss.
(Hypericaceae)
LeavesBody weight and serum glucose levels of the rats decreased. The drug also has effect on total cholesterol, triglycerides and high-density lipoprotein-cholesterol.Male Wistar rats fed with high fat diet
61Ilex paraguariensis
A.St.-Hil.
(Aquifoliaceae)
Leaves and unripe fruitsDown-regulates expression of Creb-1 andC/EBPa, and up-regulates expression of Dlk1, Gata2, Gata3, Klf2, Lrp5, Pparc2, Sfrp1,Tcf7l2, Wnt10b, and Wnt3a.The mRNA levels of PPAR-γ2 were downregulated.High fat diet- induced mice, male Wistar rats fed diet
62Ipomoea batatas(L.) Lam
(Convolvulaceae)
FruitExpression of SREBP-l, Acyl-CoA Synthase, Glycerol-3-Phosphate Acyltransferase, HMG-CoA Reductase and Fatty Acid Synthase in liver tissue in mice is altered.Mice fed with high-fat diet
63Saccharina japonica
(Phaeophyceae)
Whole PlantExpression of the fat intake-related gene ACC2 and lipogenesis-related genes are reduced. It increases phosphorylation of AMPKand its direct downstream protein,acetylcoenzyme A carboxylase.High-fat-diet-induced obese male Sprague-Dawley rats
64Larix laricina
(Du Roi) K.Koch
(Pinaceae)
Whole PlantStimulates glucose uptake, potentiated adipogenesis, activated AMPK, and acted as mitochondrial uncoupler/inhibitor (on normal isolated mitochondria).Diet-induced obese C57BL/6 mice
65Ligularia fischeri(Ledeb.) Turcz.
(Compositae)
LeavesPolyphenols present in the extract exhibits antiobesity effects by inhibiting pancreatic lipase.C57BL/6 mice
66Ligustrum lucidumW.T.Aiton
(Oleaceae)
FruitsTreatment with the extract decreases HFD-induced obesity, mainly by improving metabolic parameters, such as fats and triglycerides.High fat-diet-induced C58BL/6J obese mice
67Lithocarpus polystachyus
(Wall. ex A.DC.) Rehder
(Fagaceae).
LeavesDecreases levels of serum lipids, attenuates body weight gain and lowers circulatingleptin and insulin levels, ameliorate the state of oxidative stress, raise serum adiponectin, reduce circulating CRP and resistin levels, and depresses expression of PPARγ and C/EBPα.High fat diet-induced obese rats
68Lithospermum erythrorhizon
Siebold & Zucc.
(Boraginaceae)
RootsReduces high-fat diet-induced increases in body weight, white adipose tissue mass, serum triglyceride and total cholesterol levels, and hepatic lipid levels and decreases lipogenic and adipogenic gene expression. Acetylshikonin, active constituent ofL.erythrorhizonsuppresses adipocyte differentiation and attenuates adipogenic transcription factor expression.C57BL/6J mice were fed a normal or high-fat diet
69Morinda citrifoliaL.
(Rubiaceae)
FruitReduces body weight and fat mass. It increases glucose tolerance and reduced plasma triglycerides level.High-fat diet-induced obesity in mice
70Morus albaL.
(Moraceae)
Fruit, leavesThe hepatic peroxisome PPAR-R and carnitine palmitoyltransferase-1 are elevated, while fatty acid synthase and 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase are reduced. It decreases hepatic lipids, fatty acid synthase and 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase and elevates hepatic peroxisome PPAR-α and carnitine palmitoyltransferase-1.High fat diet- induced mice, 6-week-old male hamsters.
71Morus australisPoir
(Moraceae)
FruitReduces resistance to insulin, associated with leptin.Male C57BL/6 mice fed with high-fat diet[117]
72Morus nigraL.
(Moraceae)
Fruit, leaves,Proinflammatory cytokines MCP-1 and TNF-α, plasma triglyceride, liver lipid peroxidation levels and adipocyte size are decreased. Inflammatory markers (monocyte chemoattractant protein-1, inducible nitric oxide synthase, C-reactive protein, tumour necrosis factor-α and interleukin-1) in liver and adipose tissue are increased.Adenovirus 36-induced obesity in mice, high-fat (HF) diet-induced obese mice.[116-120]
73Murraya koenigii(L.) Spreng.
(Rutaceae)
LeavesReduces body weight gain, plasma total cholesteroland triglyceridelevels in mice.High fat diet -induced mice[121]
74Myrciaria dubia
(Kunth) McVaugh
(Myrtaceae)
FruitReduces animal body weights of the fat in white adipose tissues, glucose, total cholesterol, triglycerides, and LDL-c and insulin blood levels. An increase in HDL-c levels also seen.Wistar rats with obesity induced by subcutaneous injection of monosodium glutamate[122]
75Myrtus communisL.
(Myrtaceae)
LeavesThe body weight reduced by 32 % when administered with sibutramine, while it was reduced by 21 % and 24 % when administered with the methanolic extract ofM.communis/kg body weight.High-fat diet induced obese mice[123]
76Nelumbo nuciferaGaertn.
(Nelumbonaceae)
Seed epicarp, leaves, seed, petalsThe extracts effective in inhibiting preadipocyte differentiation. The flavonoids inhibits effect on both adipocyte differentiation and pancreatic lipase activity, accumulation and decreases expression PPARγ, GLUT4, and leptin in cultured human adipocytes, indicating that it inhibits the differentiation of pre-adipocytes into adipocytes. The methanol extract inhibitslipase activity and suppresses the expression of fatty acid synthase, acetyl-CoA carboxylase, and HMGCoA reductase and increases the phosphorylation of AMP-activated protein kinase in the liver.High fat diet- induced mice
Male Sprague-Dawley rats were fed with a normal diet and a high-fat diet,
High fat diet - induced C57BL/6 mice.
[124-127]
77Nephelium lappaceumL.
(Sapindaceae)
FruitThe expression of Igf-1 and Igf-1R were reduced on obese rat model treated with extract ofN.lappaceum.Rat fed with high calorie diet and treated with ellagic acid[128]
78Nitraria retusa(Forssk.) Asch.
(Nitrariaceae)
ShootThe extract supresses increase in body and fat mass weight, and decreases triglycerides and LDL-cholesterol levels and enhances gene expression related to lipid homeostasis in liver showing anti-obesity actions.BKS.Cg-Dock7m+/+ Leprdb/J mice model[129]
79Olea europaeaL.
(Oleaceae)
LeavesThe extract reverses HFD-induced upregulation of WNT10b- and galanin-mediated signaling molecules and key adipogenic genes (PPARγ, C/EBPα, CD36, FAS, and leptin). It also induces downregulation of thermogenic genes involved in uncoupled respiration (SIRT1, PGC1α, and UCP1) and mitochondrial biogenesis (TFAM, NRF-1, and COX2).High-fat diet-induced obesity in mice[130,131]
80Orthosiphon aristatus(Blume) Miq
(Lamiaceae)
Whole plantBetulinic acid, the active constituent suppresseshypothalamic protein tyrosine phosphatase 1B in mice and enhances the antiobesity effect of leptin in obese ratsHigh-fat-fed mice[132]
81Panax ginseng
C.A.Mey.
(Araliaceae)
RootGinsam increases PPAR- γ expression and AMP-activated protein kinase phosphorylation in liver and muscle. The extracts stronglyactivatesHormone Specific Lipasevia Protein Kinase A.Insulin - resistant rat, high fat diet induced obese C57BL6/J mice[133,134]
82Perilla frutescens
(L.) Britton
(Lamiaceae)
LeafIt decreases body weight gain, food efficiency ratio, and relative liver and epididymal fat mass.High fat diet - induced rats[135]
83Petasites japonicus
(Siebold & Zucc.) Maxim.
(Compositae)
Flower budsThe extracts attenuate three adipogenetic transcription factors,peroxisome PPAR-γ2, CCAAT/ enhancer- binding protein and sterol regulatory element- binding protein 1c.Diet- induced obesity-prone mice.[136]
84Phaseolus vulgarisL.
(Leguminosae)
BeanIt reduces food intake and body weight in an animal model of obesityresulting in suppression of glycaemia.Genetically obese adult male Zucker fa/fa rats.[137]
85Phyllostachys edulis(Carrière) J.Houz.
(Poaceae)
LeavesThe extract ameliorates elevated MCP-1 concentration in the blood.High fat diet - induced C57BL/6J mice[138]
86Pinus koraiensisSiebold & Zucc
(Pinaceae)
LeavesSuppresses fat accumulation and intracellular triglyceride associated with downregulation of adipogenic transcription factor expression, including PPARγ and CEBPα in the differentiated 3T3-L1 adipocytes. It attenuates expression of FABP and GPDH as target genes of PPARγ during adipocyte differentiation.High fat diet Male Sprague-Dawley rats[139]
87Piper fragileBenth
(Piperaceae)
SeedP.fragilerude oil shows significant reduction in body weight.Male Sprague dawley mice were treated with high cholesterol diet[140]
88Piper sarmentosumRoxb.
(Piperaceae)
leavesP.sarmentosumgroup shows reduction in enzyme activity. Aqueous extract ofP.fragilehave the ability to reduce 11β-HSD1 enzyme activity.Ovariectomy-Induced Obese Rats[141]
89Platycodon grandiflorum
(Campanulaceae)
RootsPlatycodin feeding increases cholesterol absorption up to 60%, but not cholesterol synthesis. Platycodin-enriched diets can lower circulating and whole body cholesterol contents.Golden Syrian hamsters[142]
90Polygonum aviculareL.
(Polygonaceae)
Aerial PartsExtract ofP.avicularesuppresses the elevated mRNA expression levels of sterol regulatory element-binding protein-1c, peroxisome PPAR-γ, fatty acid synthase, and adipocyte protein 2 in the white adipose tissue of obese mice.High-fat dietinduced obese mice[143]
91Populus balsamiferaL.
(Salicaceae)
Whole PlantSalicortin reduces whole body and retroperitoneal fat pad weights, as well as hepatic triglyceride accumulation. It also modulates key components in signaling pathways involved with glucose regulation and lipid oxidation in the liver, muscle, and adipose tissue.C57Bl/6 mice subjected to high fat diet[144]
92Premna integrifoliaLinn
(Verbenaceae)
RootsA significant decrease in the levels of serum glucose, triglyceride, total cholesterol, LDL and VLDL observed in the animals treated with the extract ofP.integrifolia.Female Swiss Albino mice, fed with cafetaria diet[145]
93Prunus mume(Siebold) Siebold & Zucc
(Rosaceae)
FruitIncreases CPT-1 expression and decreases FAS, ACC, and SREBP-1c in the liver and quadriceps muscles to resulting in reducing triglyceride accumulation. It also improves insulin sensitivity in OVX rats and prevents the impairment of energy, lipid, and glucose metabolism by OVX through potentiating hypothalamic leptin and insulin signaling.High fat diet, ovariectomized rats[114]
94Pueraria montanavar. chinensis (Ohwi) Sanjappa & Pradeep
(Leguminosae)
FlowerIt downregulates acetyl-CoA carboxylase expression. For adipose tissue, the expressions of hormone-sensitive lipase in white adipose tissue and uncoupling protein 1 in brown adipose tissue are upregulated.Male C57BL/6J mice were fed a high-fat diet[146]
95Punica granatumL
(Lythraceae)
Leaves, seedPunicic acid binds and activates PPAR-α and γ, thus upregulating PPAR α and its responsive genes (Stearoyl-CoA desaturase-1, SCD1; Carnitine palmitoyltransferase 1, Cpt-1; and acyl-coenzyme A dehydrogenase) as well as PPAR γ-responsive genes expression (CD36 and Fatty Acid Binding Protein4, FABP4) in intra-abdominal white adipose tissue while suppressing expression of the inflammatory cytokine TNF-α and NF-κB activation.High-fat diet induced obese mice[147,148]
96Rheum palmatumL.
(Polygonaceae)
RootIt inhibits peroxisome PPAR-γtransactivity and the expression of its target genes, suggesting that rhein acts as a PPARγ antagonist.Diet-induced obesefemale C57BL/6 mice[108,149, 150]
97Rosmarinus officinalisL.
(Lamiaceae)
LeavesIt decreases circulating tumor necrosis factor alpha, IL-1β, and leptin, and upregulated adiponectin. The extract also induces phase I and phase II gene expression and the peroxisome PPAR-γ coactivator 1-alpha. Serum triglycerides, cholesterol and insulin levels are also decreased in the lean animals.Lean (Le,fa/+) and obese (Ob,fa/fa) female Zucker rats[151]
98Rubus fruticosusL.
(Rosaceae)
FruitPurified blueberry anthocyanins have been shown to improve body weight and body composition and reduce obesity in mice.C57BL/6J
mice fed a high-fat diet
[152]
99Sapindus emarginatusVahl
(Sapindaceae)
Pericarp of flowerMethanolic extract decreases body weight, BMI, Blood glucose levels, total cholesterol, LDL-C, HDL-C, Triglycerides, SGOT, and SGPT.Monosodium glutamate induced obesity in wistar albino rats[153]
100Sasa quelpaertensisNakai
(Poaceae)
LeavesAdipogenesis is inhibited by this drug by downregulating the expression of CCAAT/enhancer-binding protein α, peroxisome PPAR-γ, SREBP-1c, and aP2. It also decreases the expression of fatty acid synthase and adiponectin mRNAs in differentiating adipocytes. It increases AMPK and acetyl-CoA carboxylasephosphorylation.High-fat diet-induced obese C57BL/6 mice[154,155]
101Schisandra chinensis(Turcz.) Baill.
(Schisandraceae)
PeelIt decreases expression of C/EBPβ,C/EBPα or PPARγ, and resultant down-regulation of the terminal marker gene, aP2 during differentiation of 3T3-L1 preadipocytes into adipocytes. Akt and GSK3β phosphorylation are down-regulated blocking adipogenesis and adipocyte differentiation.HFD-induced obese rats[156]
102Senna siamea (Lam.) H.S.Irwin & Barneby
(Leguminosae)
RootsActive constituents includes chrysophanol, physcion, emodin, cassiamin A, friedelin and cycloart-25-en-3,24- diol exhibits pancreatic lipase inhibitory activity.Alloxan induced diabetes rats.[157]
103Shorea robustaGaertn
(Diptercarpaceae)
LeavesIt decreasesserum glucose, triglyceride, cholesterol, LDL-C, HDL-C, VLDL-C, atherogenic index, SGPT and SGOT.Monosodium glutamate induced obesity in albino rats[158]
104Sida rhombifoliaL.
(Malvaceae)
LeafUp-regulation of PPARγ 2 and SREBP-1cexpression in the epididymal adipose tissue, leading to attenuation of adipogenesis.High fat diet- induced C57BL/6J mice[159]
105Solanum lycopersicumL.
(Solanaceae)
FruitAMP-activated protein kinase and acetyl-CoA carboxylase phosphorylation in liver is elevated, and HMG-CoA reductase expression is decreased. It strongly decreases expression of peroxisome PPAR-γ, CCAAT/enhancer-binding protein alpha and perilipin in the adipose tissue.High-fat-diet-induced obesity in C57BL/6 mice[160,161]
106Syzygium aromaticum(L.) Merr. & L.M.Perry
(Myrtaceae)
Flower budsThe extract suppresses expression of lipid metabolism-related proteins, including SREBP-1, FAS, CD36 and PPARγ in the liver and WAT, in addition to downregulates mRNA levels of transcription factors including SREBP and PPAR-γ.Highfatdiet fed mice[162]
107Tamarindus indicaL.
(Leguminosae)
Fruit pulp,pulp, seed coatLevelsof plasma total cholesterol, lowdensity lipoprotein, andtriglyceride is decreased and it increases high-density lipoprotein, with the concomitant reduction of body weight.Diet-induced obese Sprague-Dawley rats[163-165]
108Tecomella undulata(Sm.) Seem.BarkThe extract shows a significant increase in SIRT1 and adiponectin levels and decrease in PPAR, C/EBP, E2F1, leptin and LPL levels in preadipocytes and adipocytes and shows improvement in lipid profile and glucose levels.High Fat Diet obese mice.[166]
109Vaccinium corymbosumL.
(Ericaceae)
PeelIt inhibits lipid accumulation and decreases expression of C/EBPβ, as well as the C/EBPα and PPARγ genes during the differentiation of preadipocytes into adipocytes. It down-regulates adipocyte-specific genes such as aP2 and FAS.High-fat diet-induced obese rats[167]
110Veratrum nigrumL.
(Melanthiaceae)
Whole plantIt reduces weight gain and the fat pad weight in high fat diet-induced obese mice.High-fat diet-induced obese mice[134]
111Vigna angularis
(Willd.) Ohwi & H.Ohashi
(Leguminosae)
SeedIt reduces total hepatic lipid accumulation and lipid secretion into the feces. Incubation of adipocytes with the extract significantly decreases triglyceride accumulation, glycerol phosphate dehydrogenase activity and inflammatory responses without affecting cell viability.High fat diet-induced obesity in rats[168]
112Vigna nakashimae(Ohwi) Ohwi & H.Ohashi
(Leguminosae)
SeedsIt decreases expression of peroxisome proliferator activated receptorγ and its target genes. It enhances the phosphorylation of AMP-activated protein kinase (AMPK) and acetyl CoA carboxylase (ACC), and increases the expression of fatty acid oxidation genes.High-fat dietfed mice[169]
113Viscum albumL.
(Santalaceae)
Leaves, stems, and fruitsBody and epididymal fat pad weights are, andhistologicalexamination indicates an amelioration of fatty liver. It potently induces mitochondrial activity by activating thermogenesis and improving endurance capacity and also inhibited adipogenic factors in vitro.Male C57Bl/6 mice fed a high-fat diet[170]
114Vitis thunbergii
Siebold & Zucc
(Vitaceae)
RootsActivation of AMPK activating glucose and lipid metabolism.High-fat diet-fed C57BL/6JNarl mice[171]
115Vitis viniferaL.
(Vitaceae)
Seed flours, peel, roots, fruitBy up-regulating hepatic genes related to cholesterol (CYP51) and bile acid (CYP7A1) synthesis as well as LDL-cholesterol uptake. Lipid metabolism-associated genes Mlxp1, Stat5a, Hsl, Plin1, and Vdr were down-regulated. The extract treatment decreases expression of aP2, Fas, and Tnfa, known markers of adipogenesis, as measured by real-time polymerase reaction.Expression ofPPAR-γin liver and adipose tissue is lowered by regulating the lipid metabolism and suppressed obesity.Male Golden Syrian hamsters fed high-fat (HF) diets, high fat diet - induced C57BL/6J mice[54,171-178]
116Zanthoxylum bungeanumMaxim.
(Rutaceae)
FruitIt reduces weight gain, white adipose tissue mass, and serum triglyceride and cholesterol levels. It also decreased lipid accumulation and PPARγ, C/EBPα, SREBP-1, and FAS protein and mRNA levels in the liver.Obese C57BL/6 mice fed a high-fat diet[179]
117Ziziphus mauritianaLam
(Rhamnaceae)
BarkIt reduces body weight, fat mass and pancreatic lipase activity.High Fat Dietinduced obesity in Wistar rats[180]
118Dohaekseunggi-tangtraditional plant-based medicine(Taohe Chengqi Tang: Chinese)Commercial formulaDohaekseunggi-tangconsists of five herbs includingGlycyrrhizae uralensisFischer (40g),Rheum undulatumLinne (80g),Prunus persicaLinne (60g),Cinnamomum cassiaPresl (40g), and Natrii Sulfas (40g) mixed. The extract decreased serum total cholesterol, LDL-cholesterol,triglyceride, glucose, and leptin concentrations, and increased HDL-cholesterol and adiponectin levels and increased mRNA expression of peroxisome proliferator activated receptor-γ, uncoupling protein-2, and adiponectin in visceral adipose tissue of HFD miceHigh-fat dietinduced obese mice[181]

Table 1:Anti-obesity effect of natural occurring plants with mechanism of action studied on animal models

1Acorus calamusLinn (Araceae)Rhizome, roots and leavesEthyl acetate extract ofA. calamusinhibits α-glucosidase activity.HTT-T15 cell line[25]
2Aegle marmelosLinn (Rutaceae)LeavesActive compounds umbelliferone and esculetin depletes lipid content in the adipocytes and by decreasing the hyperlipidemia in obese rats fed with high-fat diet.3T3-L1 preadipocytes[30,31]
3Agrimonia pilosaLedeb (Rosaceae)Aerial partsActive constituent 1beta-hydroxy-2-oxopomolic acidinhibits adipocyte differentiation and expression of adipogenic marker genes, such as PPAR-γ, C/EBPalpha, GLUT4, adiponectin, aP2, ADD1/SREBP1c, resistin, and fatty acid synthase. It also inhibits adipocyte differentiation through downregulation of various adipocytokines by blocking PPAR-γ and C/EBPalpha expression.3T3-L1 preadipocytes[182]
4Alnus hirsuta(Spach) Rupr. (Betulaceae)LeavesPlatyphyllonol-5-O-β-d-xylopyranosidesuppresses the induction of PPARγ and C/EBPα protein expression, and inhibits adipocyte differentiation.3T3-L1 preadipocyte cells[183]
5Amomum cardamomumL. (Zingiberaceae)SeedsBy regulating the C/EBPα, C/EBPβ and PPARγ gene and protein expressions.3T3-L1 Cell lines.[184]
6Bauhinia variegateL. (Fabaceae)Flowers, flower buds, stem, roots, stem bark, seeds, leavesitreduces increased level of total cholesterol, triglycerides, LDLP and increases the level of HDLP, brain serotonin level. β -sitosterol in the stem induces secretion of serotonin in brain and in turn exhibits anti-obesity activity.Human neutrophils.[185]
7Brassica rapaL. (Brassicaceae)RootLipolysis-related genes includingβ3-adrenergic receptor,hormone-sensitive lipase,adipose triglyceride lipase, anduncoupling protein are induced in white adipocytes of animals treated with extract ofB. campestris. Activation of cyclic AMPK, HSL, and extracellular signal-regulated kinase are induced in EBR-treated 3T3-L1 cells.3T3 adipocytes.[52]
8Caesalpinia sappanL. (Leguminosae)HeartwoodBrazilein inhibits intracellular lipid accumulation during adipocyte differentiation in 3T3-L1 cells and suppresses the induction of peroxisome PPAR-γ (PPARγ).Postconfluent 3T3-L1 preadipocytes[186]
9Citrus aurantiumL. (Rutaceae)Fruits, leavesIt inhibits Akt activation and GSK3β phosphorylation, which induces the down-regulation of lipid accumulation and lipid metabolizing genes, ultimately inhibiting adipocyte differentiation.3T3-L1 preadipocytes[22,187,188]
10Coptis chinensisFranch. (Ranunculaceae)RhizomeIt inhibits lipid accumulation in 3T3-L1 cells. The five alkaloids present in this plant significantly reduces expression levels of several adipocyte marker genes including proliferator activated receptor and CCAAT/enhancer- binding protein. Isolated alkaloids found to inhibitadipogenesis.3T3-L1 adipocytes cells[189]
11Cucurbita moschataDuchesne (Cucurbitaceae)StemsReduces expression of peroxisome PPAR-γ, CCAAT/enhancer-binding protein α, fatty acid-binding protein 4, sterol response element-binding protein-1cand stearoyl-coenzyme A desaturase-1, and decreases lipid accumulation.Primary mouse embryonic fibroblasts[190]
12Curcuma longaL. (Zingiberaceae)RhizomesIncrease hormone-sensitive lipase and adipose triglyceride lipase mRNA levels and decreases perilipin mRNA level via AMPK, resulting in lipolysis.In adipose tissue, curcumin inhibits macrophage infiltration and nuclear factor κB activation induced by inflammatory agents.3T3-L1 adipocytes[191,192]
13Cyclopia falcata(Harv.) Kies (Leguminosae)StemFlavonoid, phloretin-3′,5′-di-C-glucosideinhibits intracellular triglyceride and down regulates PPAR2 expression and inin vitrocondition it can inhibit adipogenesis.3T3-L1 mouse pre-adipocytes[193]
14Cyclopia maculata(Andrews) Kies (Leguminosae)StemsMangiferin, hesperidin inhibits intracellular triglyceride and fat accumulation, and decreases PPAR2 expression and inin vitroit can inhibit adipogenesis.3T3-L1 mouse pre-adipocytes[193]
15Dalbergia sissooDC. (Leguminosae)LeavesInhibits pancreatic lipase and can be used as an anti-obesity agent in suitable form.Chicken pancreas[194]
16Dioscorea oppositifoliaL. (Dioscoreaceae)RhizomeDecreases expression of PPAR-γ. Batatasin I compound from the extract was found to increase p-AMPK and CPT-1 in 3T3-L1 adipocytes, resulting in inhibiting adipogenesis.Mouse embryo preadipocyte (3T3-L1) cell lines[195]
17Eremochloa ophiuroides(Munro) Hack (Poaceae)Whole PlantExpression of C/EBP and PPAR, the central transcriptional regulators of adipogenesis. Moreover, this plant down-regulates phosphorylation levels of Akt and GSK3.Mouse 3T3-L1 preadipocytes[196]
18Glycine max(L.)Merr. (Leguminosae)BeanIt inhibits adipocyte differentiation in 3T3-L1 preadipocyte cells. Accumulation of triglycerides is inhibited and activation of AMPK.3T3-L1 preadipocyte cells[89,90]
19Houttuynia cordataThunb. (Saururaceae)LeafAttenuates expression of fatty acid synthase, sterol regulatory element-binding protein-1 and glycerol 3-phosphate acyltransferases. The extract inhibits the elevation of plasma TG levels in mice. The extractspossibly suppress the uptake of NEFA and glycerol by blocking FAT/CD 36 and also suppress aquaproin-7.Human HepG2 hepatocytes[197,198]
20Ilex paraguariensisA.St.-Hil. (Aquifoliaceae)Leaves and unripe fruitsA modulatory effect on the expression of genes related to the adipogenesis as PPAR2, leptin, TNF and C/EBP are also seen.3T3-L1 cell line[102-105]
21Ipomoea batatas(L.) Lam (Convlvulaceae)FruitExpression of SREBP-l, Acyl-CoA Synthase, Glycerol-3-Phosphate Acyltransferase, HMG-CoA Reductase and Fatty Acid Synthase in liver tissue.Murine 3T3-LI adipocytes[106]
22Irvingia gabonensis(Aubry-Lecomte ex O'Rorke) Baill. (Irvingiaceae)SeedInhibits adipogenesis in adipocytes. The effect appears to be mediated through the down regulated expression of adipogenic transcription factors (PPAR-γ)and adipocyte-specific proteins (leptin), and by upregulated expression of adiponectin.Murine 3T3-LI adipocytes[199]
23Morus australisPoir. (Moraceae)RootIncreases lipolytic effects such as decreased intracellular triglyceride and the release of glycerol.3T3-L1 adipocytes[200]
24Nelumbo nuciferaGaertn. (Nelumbnaceae)Seed epicarp, leaves, seed, petalsThe extracts effective in inhibiting preadipocyte differentiation. The flavonoids inhibits effect on both adipocyte differentiation and pancreatic lipase activity, accumulation and decreases expression PPARγ, GLUT4, and leptin in cultured human adipocytes, indicating that it inhibits the differentiation of pre-adipocytes into adipocytes.3T3-L1 (adipocyte), NIH3T3 (mouse fibroblast, embryo), L-02 (normal hepatocyte) cells CHO-K1, and U2OS cells,[124-127]
25Nepeta tenuifoliaBenth. (Lamiaceae)Whole plantInhibits triglyceride accumulation in 3T3-L1 adipocytes, suggesting anti-obesity activity.3T3-L1 cells[201]
26Pericarpium zanthoxyli(Rutaceae)SeedDecreases expression of the adipogenesis-related transcription factor,PPAR-γ and PPAR-γ-target genes, such as adipocyte protein 2 (aP2), fatty acid synthase (FAS) and other adipocyte markers and also decreases levels of CCAAT/enhancer-binding protein β (C/EBPβ) in a dose-dependent manner.OP9 cells[202]
27Petasites japonicus(Siebold & Zucc.)Flower budsThe extracts attenuate three adipogenetic transcription factors,peroxisome PPAR-γ2, CCAAT/ enhancer- binding protein and sterol regulatory element- binding protein 1c.3T3-L1 murine preadipocytes[136]
28Peucedanum japonicumThunb. (Apiaceae)LeavesPteryxin down regulates genes SREBP-1c, fatty acid synthase, and acetyl-coenzyme A carboxylase-1 in treated 3T3-L1 adipocytes and HepG2 hepatocytes and up-regulates lipid catabolizing genes. In aother study it was proved that the extract down-regulates a key lipogenic activator, SREBP1 c and adipocyte size marker gene, paternally expressed gene 1/mesoderm-specific transcript (PEG1/MEST) in adipose tissue in vivo.Both 3T3-L1 and HepG2 cell lines, 3T3-L1 and HepG2 cells[203,204]
29Rubus chingiivar. suavissimus (S.K.Lee) L.T.Lu (Rosaceae).LeavesThe extract increases adipogenesis and increases expression of adiponectin and leptin. In the early phase of adipogenesis, extract increases the mRNA expression of adipogenic transcription factors CCAAT/enhancer binding protein α and PPAR-γ.3T3-L1 preadipocytes[205]
30Salicornia herbaceaL. (Amaranthaceae)Whole plantIsorhamnetin 3-О-β-D-glucopyranoside suppresses adipogenic differentiation by downregulation of peroxisome proliferator-activated receptor-γ, CCAAT/enhancer-binding proteins, SREBP1, and the adipocyte-specific proteins. Specific mechanism mediating the effects of isorhamnetin 3-О-β-D-glucopyranoside confirmed by activation of AMPK.3T3-L1 preadipocytes[206]
31Siegesbeckia pubescensL. (Amaranthaceae)Whole plantThe anti-obesity effect is modulated by cytidine-cytidine-adenosine-adenosine-thymidine/enhancer binding proteins, and peroxisome proliferator-activated receptor, gene and protein expressions.3T3-L1 preadipocytes[207]
32Smilax chinaL. (Smilacaceae)LeavesPolyphenol and flavonoid, exhibits α-glucosidase and lipid accumulation inhibition properties.3T3-L1 adipocytes[208]
33Tetrapanax papyriferus(Hook.) K.Koch (Araliaceae)Whole plantThe anti-obesity effect is modulated by cytidine-cytidine-adenosine-adenosine-thymidine/enhancer binding proteins, and peroxisome proliferator-activated receptor, gene and protein expressions.3T3-L1 preadipocytes[207]
34Veratrum nigrumL. (Melanthiaceae)Whole plantIt decreases lipid accumulation and the expressions of two major adipogenesis factors, PPAR and C/EBP, in 3T3-L1 cells.3T3-L1 cells[134]
35Vitis labruscaL. (Vitaceae)SeedThe extract ofV.labruscainhibits lipid accumulation of C3H10T1/2 and 3T3-L1 cells in a dose-dependent manner. Inhibition is associated with reduced expression of PPAR-γ.C3H10T1/2 and 3T3-L1 cells[177]
36Vitis viniferaL. (Vitaceae)Seed flours, peel, roots, fruitBy up-regulating hepatic genes related to cholesterol (CYP51) and bile acid (CYP7A1) synthesis as well as LDL-cholesterol uptake. Lipid metabolism-associated genes Mlxp1, Stat5a, Hsl, Plin1, and Vdr were down-regulated. The extract treatment decreases expression of aP2, Fas, and Tnfa, known markers of adipogenesis, as measured by real-time polymerase reaction.Expression ofPPAR-γin liver and adipose tissue is lowered by regulating the lipid metabolism and suppressed obesity.3T3-L1 preadipocytes, high fat diet- induced mice, murine 3T3-LI adipocytes,[54,171-178]
37Ziziphus jujubeMill. (Rhamnaceae)FruitSuppresses lipid accumulation and glycerol-3-phosphate dehydrogenase. Elicits the most inhibitory effect with attenuation of the expression of key adipogenic transcription factors, includingPPAR-γ and CCAAT enhancer binding proteins (C/EBPs) at the protein level.3T3-L1 preadiocytes[209-211]
38Germinated brown rice, germinated waxy brown rice, germinated black rice, and germinated waxy black riceSeedExtract of these seeds decreases body weight gain and lipid accumulation in the liver and epididymal adipose tissue. The mRNA levels of adipogenic transcriptional factors, such as CCAAT enhancer binding protein (C/EBP)-α, SREBP(SREBP)-1c, and peroxisome proliferator activated receptors (PPAR)-γ, and related genes (aP2, FAS) are decreased by the seed extract.3T3-L1 murine adipocytes[212]

Table 2:Anti-obesity effect of plants with mechanism of action studied on cell line as model.

1Carum carviL. (Apiaceae)SeedReduces weight, body mass index, body fat percentage, and waist-to-hip ratio.Human clinical trials[213]
2Cissus quadrangularisL. (Vitaceae)Cylaris a formula contains aC.quadrangularextractPhytosterols and fiber extracts have anti-lipase, and anorexiant properties that reduce the absorption of dietary fats and enhance satiation by increasing serum serotonin levels.Human clinical trials[214]
3Citrus aurantiumL. (Rutaceae)Fruits, leavesThe active constituent p-synephrine increases metabolic rate, energy expenditure and increase in weight loss. The leaf extract down-regulates the expression of C/EBPβ and subsequently inhibits the activation of PPARγ and C/EBPα. The anti-adipogenic activity of is mediated by the inhibition of Akt activation and GSK3β phosphorylation, which induces the down-regulation of lipid accumulation and lipid metabolizing genes, ultimately inhibiting adipocyte differentiation.Human clinical trials[22,187,188]
4Garcinia cowaRoxb. ex Choisy (Clusiaceae)Fruit, commercially available tabletInhibits the enzyme ATP-dependent citrate lyase, which catalyzes the cleavage of citrate to oxaloacetate and acetyl-CoA.Serum apo A1 levels are increased by the plant and the serum total cholesterol levels.Human clinical trials[86,87]
5Gynostemma pentaphyllum(Thunb.) Makino (Cucurbitaceae)LeavesActivation of AMPK by 5-aminoimidazole-4-carboxamide- 1-b-D-ribofuranoside (AICAR) inhibits adipogenesis by downregulating PPARcand CEBP1a as well as lipogenic factors including fatty acid binding protein 4 and lipoprotein lipase. AMPK activation directly inactivates ACC through Ser79 phosphorylation, leading to decreased fat synthesis by reducing the production of malonyl-CoA from acetyl-CoA.Human clinical trials[215]
6Nigella sativaL. (Ranunculaceae)CommercialNigella sativaoil prepared by steam distillation.N.sativareduces total cholesterol, low density lipoprotein (LDL) and fasting blood glucose. The oil is effective as an add-on therapy in patients with metabolic syndrome.Human Volunteer[216,217]
7Salacia reticulatawight (Celastraceae)RootSignificant weight and body-fat reduction was observed inS.reticulatatreated animals and also BMI reduction is seen.Human clinical trials[218]
8Trigonella foenum-graecumL. (Leguminosae)SeedDaily fat consumption, expressed as the ratio fatreported energy intake/total energy expenditure (fat-REI/ TEE), is decreased in overweight subjects administered the fenugreek seed extract. Significant decrease in the insulin/glucose ratio in subjects treated with fenugreek seed extract.Clinical trials[219]
9Turnera diffusaWilld. ex Schult. (Passifloraceae)LeavesThe herbal preparation capsules delays gastric emptying, reducing the time to perceived gastric fullness and induces weight loss.Healthy volunteers[220,221]
10Vernonia amygdalinaDelile (Compositae)LeafFat eliminationusually occurred within 2 sec, 2 min of extract intake. The blood glucose lowers effects ofV. amygdalinaleaf extract were usually exerted in 2 sec, 2 min of extract intake by the patient.Clinical trials[222]
11Ziziphus jujubeMill. (Rhamnaceae)FruitThe extract suppresses lipid accumulation and glycerol-3-phosphate dehydrogenase.Z. jujubaextract elicits the most inhibitory effect with attenuation of the expression of key adipogenic transcription factors, includingPPAR-γ and CCAAT enhancer binding proteins (C/EBPs) at the protein level.Human clinical trials[209-211]

Table 3:List of plants exhibiting anti-obesity activity studied on human volunteers.

1.Verbesina persicifoliaDC (Compositae)Aerial parts4β-cinnamoyloxy,1β,3α-dihydroxyeudesm-7,8-ene is the active constituent present inV.persicifoliainduces bioenergetic collapse in rat liver mitochondria, demonstrating typical uncoupling agent. It acts as a mild uncoupler droping Δψ and increases respiratory state 4. The energy collapse, mild uncoupling, and the fact thatV.persicifoliais largely used in folk medicines, this plant may be viewed as a potentially effective anti-obesity drug.Rat liver mitochondria[223]

Table 4:Anti-obesity effect of plants with mechanism of action studied on isolated cell organelles.

1.Fraxinus chinensissubsp. rhynchophylla (Hance) A.E.Murray(Oleaceae)Stems and barksMajor active components are secoiridoids ligstroside, oleuropein, 2"-hydroxyoleuropein and hydroxyframoside B. These compounds significantly inhibit pancreatic lipase and hydroxyframoside B being the most active inhibitor in a mixed mechanism of competitive and noncompetitive manner.Porcine pancreatic lipase[224]
2Vitis viniferaL.(Vitaceae)Seed flours, peel, roots, fruitBy up-regulating hepatic genes related to cholesterol (CYP51) and bile acid (CYP7A1) synthesis as well as LDL-cholesterol uptake. Lipid metabolism-associated genes Mlxp1, Stat5a, Hsl, Plin1, and Vdr were down-regulated. The extract treatment decreases expression of aP2, Fas, and Tnfa, known markers of adipogenesis, as measured by real-time polymerase reaction.Expression ofPPAR-γin liver and adipose tissue is lowered by regulating the lipid metabolism and suppressed obesity.Pure pancreatic lipase[54,171-178]

Table 5:Anti-obesity effect of plants with mechanism of action studied on isolated cell enzymes.

Discussion

In this review, we have report the antiobesity effects of different herbal plants or compounds containing minerals or chemical extracts of plants. Plants having reported antiobesity effects are listed inTable 1with information about their active components and their effects. From the review it was suggested that, plant showing anti-obesity potential mainly belongs to the family Leguminoseae, Lamiaceae, Liliaceae, Cucurbitaceae, Asteraceae, Moraceae, Rosaceae and Araliaceae. Majority of the studies indicates decrease in body weight or body weight gain in animals and humans with or without changes in body fat indicating antiobesity effects. The antiobesity effects such as body weight reduction, decrease in the levels of triglycerides, total cholesterol, and low density lipoprotein cholesterol with simultaneous increase in high density lipoprotein cholesterol was observed in the animals treated with the plants [1,15,29,31,39,54,60,78,79,83,85,98,100,101,133,145,152,165,190,196,201]. In one study [41], it has been reported that a compound chakasaponin II, suppressed mRNA levels of neuropeptide Y (NPY) and enhanced the release of serotonin (5-HT) that suppressed the appetite signals in the hypothalamus of the mice. Clinical trials were conducted on humans for various plant extracts [45,49,135] which showed a significant decrease in body weight and body fat reduction. There was an increase in metabolic rate and energy expenditure. It was also reported that the clinical trials performed on humans for the plant extracts [151,165,226] showed an excess fat elimination, body mass index, fat percentage and blood glucose lowering effects. In another clinical trial study [148] the fenugreek seed extract decreased the fat consumption and also insulin/glucose ratio. The essential oil from the plants [124,227] suppressed fat accumulation, intracellular triglyceride and decrease in body weight. Ginseng which is a popular Chinese herbal medicine significantly decreased the weight gain and improved glucose tolerance [115,120].P. granatumexhibits potential antiobesity mechanism including inhibition of pancreatic lipase activity and suppression of energy intake. Its effect on energy intake was similar to subutramine but with a different mechanism.

A study reported that Green tea possessed higher antioxidant activity than antiobesity activity due to its high concentration of catechins, including epicatechins, ECG and EGCG. It was proved that antiobesity activity of catechins resulted from the combined actions of appetite reduction, greater lipolytic activity, energy expenditure and adipocyte differentiation.

The active compounds umbelliferone and esculetin from the plantAegle marmeloshave shown marked effect by depleting the lipid content in the adipocytes and by decreasing the hyperlipidemia. Similarly, galangin a compound fromAlpinia galangalshowed a significant decrease in serum lipids, liver weight, lipid peroxidation and accumulation of hepatic Triglycerides. Decursin a compound fromAngelica gigassignificantly improved glucose tolerance and reduced the secretion of HFD-induced adipocytokines. The phytoconstituent compound sitosterol found inBoerhaavia diffusais structurally similar to cholesterol has been suggested to reduce cholesterol by lowering the level of LDL-cholesterol. p-synephrine compound from the plantCitrus aurantiumshowed increased metabolic rate, energy expenditure and increase in weight loss. InNelumbo nuciferaflavonoids showed mild inhibitory effect on both adipocyte differentiation and pancreatic lipase activity. Among the flavonoids, flavones without glucose inhibited pancreatic lipase activity, whereas flavone glycosides did not show inhibition. The presence of ephedrine and pseudoephedrine in the plantSida rhomboideainduced appetite suppression that inhibits body weight gain.

Conclusion

Natural products identified from traditional medicinal plants have always paved the way for development of new types of therapeutics. Generally most of the compounds were isolated from natural sources despite which orlistat a semi-synthetic derivative of lipstatin have been approved by the US food and drug administration for the treatment of obesity. Orlistat is a potent inhibitor of pancreatic lipase (PL) which is a lipolytic enzyme which hydrolyses dietary fats in the initial step of lipid metabolism. There have been many reports on other effects such as anti-oxidative stress effects which may be important in the management of other diseases like cardiovascular diseases and diabetes. The antiobesity drugs are generally preferred based on high efficacy and effectiveness. The active exploration of natural sources has provided new developments based on the understanding of complex and redundant physiological mechanisms. Such exploration will lead to a safe and effective pharmacological treatment.

References

 

Plants and their phytonutrients have a lot of potential as anti-obesity agents.

Admin

Reply
Share: